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Tim Stasevich
Tim Stasevich Associate Professor

Office: MRB 285

Phone: (970) 491-3250

Website: https://stasevichlab.colostate.edu

Google Scholar: https://scholar.google.com/citations?user=sGlZEDEAAAAJ&hl=en

Education

  • Ph.D., University of Maryland

About

Timothy J. Stasevich is an Assistant Professor in the Department of Biochemistry at Colorado State University (CSU). His lab uses a combination of advanced fluorescence microscopy, genetic engineering, and computational modeling to study the dynamics of gene regulation in living mammalian cells. Most recently, his lab has pioneered the imaging of real-time single mRNA translation dynamics in living cells [1]. Dr. Stasevich received his B.S. in Physics and Mathematics from the University of Michigan, Dearborn, and his Ph. D. in Physics from the University of Maryland, College Park. He transitioned into experimental biophysics as a post-doctoral research fellow in the laboratory of Dr. James G. McNally at the National Cancer Institute. During this time, he developed technology based on fluorescence microscopy to help establish gold-standard measurements of live-cell protein dynamics. Dr. Stasevich next moved to Osaka University, where he worked with Dr. Hiroshi Kimura as a Japan Society for the Promotion of Science Foreign Postdoctoral Research Fellow. While there, he helped create technology to image endogenous proteins and their post-translation modifications in vivo. This allowed him to image the live-cell dynamics of epigenetic histone modifications during gene activation for the first time [2]. Before joining the faculty at CSU, Dr. Stasevich took a one year hiatus at the HHMI Janelia Research Campus, where he further improved the spatio-temporal resolution of endogenous protein imaging in live-cells.

 

1.         Morisaki, T. et al. Real-time quantification of single RNA translation dynamics in living cells. Science 352, 1425–1429 (2016).

2.         Stasevich, T. J. et al. Regulation of RNA polymerase II activation by histone acetylation in single living cells. Nature 516, 272–275 (2014).

 

Publications

  • Using Mechanistic Models and Machine Learning to Design Single-Color Multiplexed Nascent Chain Tracking Experiments.Will Scott Raymond, Sadaf Ghaffari, L U. Aguilera, Eric Ron, Tatsuya Morisaki, Zach Russell Fox, Michael Patrick May, Tim John Stasevich, Brian Munsky bioRxiv : the preprint server for biology, 2023.
  • Visualization, Quantification, and Modeling of Endogenous RNA Polymerase II Phosphorylation at a Single-copy Gene in Living Cells.L S. Forero-Quintero, Will Scott Raymond, Brian Munsky, Tim John Stasevich Bio-protocol, 15, 2022.
  • Imaging translational control by Argonaute with single-molecule resolution in live cells.Charlotte Ayn Cialek, Gabriel Galindo, Tatsuya Morisaki, Ning Zhao, Tai Montgomery, Tim John Stasevich Nature communications, 1, 2022.
  • A Drosophila toolkit for HA-tagged proteins unveils a block in autophagy flux in the last instar larval fat body.T Murakawa, T Nakamura, K Kawaguchi, F Murayama, Ning Zhao, Tim John Stasevich, H Kimura, N Fujita Development (Cambridge, England), 6, 2022.
  • Single-Molecule Imaging of mRNA Interactions with Stress Granules.Tatsuya Morisaki, Tim John Stasevich Methods in molecular biology (Clifton, N.J.), 2022.
  • Generation and diversification of recombinant monoclonal antibodiesKeith F. DeLuca, Jeanne E. Mick, A. H. Ide, W. C. Lima, L. Sherman, K. L. Schaller, S. M. Anderson, Ning Zhao, Tim John Stasevich, D. Varma, J. Nilsson, Jennifer G. DeLuca Elife, 2021.
  • Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells.Yali Liu, Ning Zhao, M T. Kanemaki, Y Yamamoto, Y Sadamura, Y Ito, M Tokunaga, Tim John Stasevich, H Kimura Genes to cells : devoted to molecular & cellular mechanisms, 11, 2021.
  • A Multi-color Bicistronic Biosensor to Compare the Translation Dynamics of Different Open Reading Frames at Single-molecule Resolution in Live Cells.Amanda Lynn Koch, Tatsuya Morisaki, Tim John Stasevich Bio-protocol, 14, 2021.
  • Bead Loading Proteins and Nucleic Acids into Adherent Human Cells.Charlotte Ayn Cialek, Gabriel Galindo, Amanda Lynn Koch, Matt Neeley Saxton, Tim John Stasevich Journal of visualized experiments : JoVE, 172, 2021.
  • Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene.L S. Forero-Quintero, Will Scott Raymond, T Handa, Matt Neeley Saxton, Tatsuya Morisaki, H Kimura, E Bertrand, Brian Munsky, Tim John Stasevich Nature communications, 1, 2021.
  • Protein manipulation using single copies of short peptide tags in cultured cells and in Drosophila melanogaster.M A. Vigano, C M. Ell, Kustermann MMM, G Aguilar, S Matsuda, Ning Zhao, Tim John Stasevich, M Affolter, G Pyrowolakis Development (Cambridge, England), 6, 2021.
  • Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single geneLinda Stephanie Forero, Will Scott Raymond, Tetsuya Handa, Matthew Saxton, Tatsuya Morisaki, Hiroshi Kimura, Edouard Bertrand, Brian Munsky, Tim John Stasevich bioRxiv, 2020.
  • Author Correction: Quantifying the dynamics of IRES and cap translation with single-molecule resolution in live cells.Amanda Lynn Koch, L Aguilera, Tatsuya Morisaki, Brian Munsky, Tim John Stasevich Nature structural & molecular biology, 12, 2020.
  • Quantifying the dynamics of IRES and cap translation with single-molecule resolution in live cells.Amanda Lynn Koch, L Aguilera, Tatsuya Morisaki, Brian Munsky, Tim John Stasevich Nature structural & molecular biology, 12, 2020.
  • Lamin A/C Deficiency Enables Increased Myosin2 Bipolar Filament Ensembles Which Promote Divergent Actomyosin Network AnomaliesO'Neil P. Wiggan, Jennifer G. DeLuca, Tim John Stasevich, James R. Bamburg Molecular Biology of the Cell, 21, 2020.
  • Coupling of translation quality control and mRNA targeting to stress granules.Shaun Moon, Tatsuya Morisaki, Tim John Stasevich, R Parker The Journal of cell biology, 8, 2020.
  • Lighting up single-mRNA translation dynamics in living cells.Charlotte Ayn Cialek, Amanda Lynn Koch, Gabriel Galindo, Tim John Stasevich Current opinion in genetics & development, 2020.
  • A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivoNing Zhao, Kouta Kamijo, Philip Douglas Fox, Haruka Oda, Tatsuya Morisaki, Yuko Sato, Hiroshi Kimura, Tim John Stasevich NATURE COMMUNICATIONS, 2019.
  • Computational design and interpretation of single-RNA translation experimentsLuis U. Aguilera, William Raymond, Zachary R. Fox, Michael Patrick May, Elliot Djokic, Tatsuya Morisaki, Tim John Stasevich, Brian Munsky PLOS COMPUTATIONAL BIOLOGY, 10, 2019.
  • Live-Cell Single RNA Imaging Reveals Bursts of Translational FrameshiftingKenneth Ray Lyon, Luis U. Aguilera, Tatsuya Morisaki, Brian Munsky, Tim John Stasevich MOLECULAR CELL, 1, 2019.
  • Multicolour single-molecule tracking of mRNA interactions with RNP granulesStephanie L. Moon, Tatsuya Morisaki, Anthony Khong, Kenneth Ray Lyon, Roy Parker, Tim John Stasevich NATURE CELL BIOLOGY, 2, 2019.
  • Satb1 integrates DNA binding site geometry and torsional stress to differentially target nucleosome-dense regionsRajarshi P. Ghosh, Quanming Shi, Linfeng Yallg, Michael P. Reddick, Tatiana Nikitina, Victor B. Zhurkin, Polly Fordyce, Tim John Stasevich, Howard Y. Chang, William J. Greenleaf, Jan T. Liphardt NATURE COMMUNICATIONS, 2019.
  • Probing Cytoskeletal mechanics and heterogeneities using glass-bead-loading to deliver 100 nm fluorescent particles into mammalian cellsWenlong Xu, Tim John Stasevich, Ashok Prasad Soft Matter.
  • Quantifying single-mRNA translation kinetics in living cellsTim John Stasevich, Tatsuya Morisaki, Cold Springs Harbor Perspectives in Biology, 11, 2018.
  • Visualizing the Dynamics of Inactive X Chromosomes in Living Cells Using Antibody-Based Fluorescent ProbesYuko Sato, Tim John Stasevich, Hiroshi Kimura, X-Chromosome Inactivation: Methods in Molecular Biology, 2018.
  • Imaging Translational and Post-Translational Gene Regulatory Dynamics in Living Cells with Antibody-Based ProbesKenneth Ray Lyon, Tim John Stasevich TRENDS IN GENETICS, 5, 2017.
  • She1 affects dynein through direct interactions with the microtubule and the dynein microtubule-binding domainKari Hope Ecklund, Tatsuya Morisaki, Lindsay G. Lammers, Matthew George Marzo, Tim John Stasevich, Steven M. Markus Nature Communications, 1, 2017.
  • Real-time quantification of single RNA translation dynamics in living cellsTatsuya Morisaki, Kenneth Ray Lyon, Keith F. DeLuca, Jennifer G. DeLuca, Brian P. English, Zhengjian Zhang, Luke D. Lavis, Jonathan B. Grimm, Sarada Viswanathan, Loren L. Looger, Timothee Lionnet, Tim John Stasevich Science, 6292, 2016.
  • Estimating cellular parameters through optimization procedures: elementary principles and applicationsAkatsuki Kimura, Antonio Celani, Hiromichi Nagao, Tim John Stasevich, Kazuyuki Nakamura Frontiers in physiology, 2015.
  • High-performance probes for light and electron microscopySarada Viswanathan, Megan E. Williams, Erik B. Bloss, Tim John Stasevich, Colenso M. Speer, Aljoscha Nern, Barret D. Pfeiffer, Bryan M. Hooks, Wei-Ping Li, Brian P. English, others Nature methods, 6, 2015.
  • Visualizing posttranslational and epigenetic modifications of endogenous proteins in vivoHiroshi Kimura, Yoko Hayashi-Takanaka, Tim John Stasevich, Yuko Sato Histochemistry and cell biology, 2, 2015.
  • Evaluation of chemical fluorescent dyes as a protein conjugation partner for live cell imagingYoko Hayashi-Takanaka, Tim John Stasevich, Hitoshi Kurumizaka, Naohito Nozaki, Hiroshi Kimura PloS one, 9, 2014.
  • Quantifying histone and RNA polymerase II post-translational modification dynamics in mother and daughter cellsTim John Stasevich, Yuko Sato, Naohito Nozaki, Hiroshi Kimura Methods, 2, 2014.
  • Regulation of RNA polymerase II activation by histone acetylation in single living cellsTim John Stasevich, Yoko Hayashi-Takanaka, Yuko Sato, Kazumitsu Maehara, Yasuyuki Ohkawa, Kumiko Sakata-Sogawa, Makio Tokunaga, Takahiro Nagase, Naohito Nozaki, James G. McNally, others Nature, 7530, 2014.
  • Convergence of chromatin binding estimates in live cellsDavide Mazza, Florian Mueller, Tim John Stasevich, James G. McNally Nature methods, 8, 2013.
  • Genetically encoded system to track histone modification in vivoYuko Sato, Masanori Mukai, Jun Ueda, Michiko Muraki, Tim John Stasevich, Naoki Horikoshi, Tomoya Kujirai, Hiroaki Kita, Taisuke Kimura, Seiji Hira, others Scientific reports, 2013.
  • Quantifying transcription factor kinetics: at work or at play?Florian Mueller, Tim John Stasevich, Davide Mazza, James G. McNally Critical reviews in biochemistry and molecular biology, 5, 2013.
  • Single-cell in vivo analysis reveals that relocation of the yeast CUP1 locus to the nuclear pore is associated with its mRNA releaseYEASTTatiana Karpova, RS Kent, Min J. Kim, F Mueller, Tim John Stasevich, D Mazza, J McNally Yeast, 2013.
  • Monitoring dynamic binding of chromatin proteins in vivo by fluorescence correlation spectroscopy and temporal image correlation spectroscopyDavide Mazza, Tim John Stasevich, Tatiana S. Karpova, James G. McNally Chromatin Remodeling: Methods and Protocols, 2012.
  • A selection and analysis tool for fluorescence recovery after photobleaching (FRAP) region of interest (ROI)Tim JohnStasevich Wolfram Demonstration Project, 2011.
  • Assembly of the transcription machinery: ordered and stable, random and dynamic, or both?Tim John Stasevich, James G. McNally Chromosoma, 6, 2011.
  • Highly stable loading of Mcm proteins onto chromatin in living cells requires replication to unloadMarjorie A. Kuipers, Tim John Stasevich, Takayo Sasaki, Korey A. Wilson, Kristin L. Hazelwood, James G. McNally, Michael W. Davidson, David M. Gilbert The Journal of cell biology, 1, 2011.
  • Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labelingYoko Hayashi-Takanaka, Kazuo Yamagata, Teruhiko Wakayama, Tim John Stasevich, Takashi Kainuma, Toshiki Tsurimoto, Makoto Tachibana, Yoichi Shinkai, Hitoshi Kurumizaka, Naohito Nozaki, others Nucleic acids research, 2011.
  • Core histone hyperacetylation impacts cooperative behavior and high-affinity binding of histone H1 to chromatinNikhil Raghuram, Gustavo Carrero, Tim John Stasevich, James G. McNally, John Th’ng, Michael J. Hendzel Biochemistry, 21, 2010.
  • Cross-validating FRAP and FCS to quantify the impact of photobleaching on in vivo binding estimatesTim John Stasevich, Florian Mueller, Ariel Michelman-Ribeiro, Tilman Rosales, Jay R. Knutson, James G. McNally Biophysical journal, 9, 2010.
  • Dissecting the binding mechanism of the linker histone in live cells: an integrated FRAP analysisTim John Stasevich, Florian Mueller, David T. Brown, James G. McNally The EMBO journal, 7, 2010.
  • Epitaxial growth writ largeTheodore L. Einstein, Tim John Stasevich Science, 5964, 2010.
  • FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know?Florian Mueller, Davide Mazza, Tim John Stasevich, James G. McNally Current opinion in cell biology, 3, 2010.
  • Direct measurement of association and dissociation rates of DNA binding in live cells by fluorescence correlation spectroscopyAriel Michelman-Ribeiro, Davide Mazza, Tilman Rosales, Tim John Stasevich, Hacene Boukari, Vikas Rishi, Charles Vinson, Jay R. Knutson, James G. McNally Biophysical journal, 1, 2009.
  • Effects of impurities on surface morphology: some examplesAjmi BH Hamouda, Tim John Stasevich, Alberto Pimpinelli, TL Einstein Journal of Physics: Condensed Matter, 8, 2009.
  • Effects of impurities on surface morphology: some examples.Hamouda A. Bh, Tim John Stasevich, A Pimpinelli, TL Einstein Journal of physics. Condensed matter: an Institute of Physics journal, 8, 2009.
  • Impurity decoration for crystal shape control: C 60 on Ag (111)Tim John Stasevich, Chenggang Tao, William G. Cullen, Ellen D. Williams, TL Einstein Physical review letters, 8, 2009.
  • Concurrent fast and slow cycling of a transcriptional activator at an endogenous promoterTatiana S. Karpova, Min J. Kim, Corentin Spriet, Kip Nalley, Tim John Stasevich, Zoulika Kherrouche, Laurent Heliot, James G. McNally Science, 5862, 2008.
  • Sensitivity of short-range trio interactions to lateral relaxation of adatoms: Challenges for detailed lattice-gas modelingRajesh Sathiyanarayanan, Tim John Stasevich, TL Einstein Surface Science, 6, 2008.
  • Step line tension and step morphological evolution on the Si (111)(1$$ 1) surface!!!AB Pang, KL Man, MS Altman, Tim John Stasevich, F Szalma, TL Einstein Physical Review B, 11, 2008.
  • Analytic formulas for the orientation dependence of step stiffness and line tension: Key ingredients for numerical modelingTim John Stasevich, TL Einstein Multiscale Modeling & Simulation, 1, 2007.
  • Facet-edge fluctuations with periphery diffusion kineticsM Degawa, Tim John Stasevich, A Pimpinelli, TL Einstein, ED Williams Surface Science, 18, 2007.
  • Metal-molecule interface fluctuationsChenggang Tao, Tim John Stasevich, William G. Cullen, TL Einstein, Ellen D. Williams Nano letters, 6, 2007.
  • Distinctive fluctuations in a confined geometryM Degawa, Tim John Stasevich, WG Cullen, Alberto Pimpinelli, TL Einstein, ED Williams Physical review letters, 8, 2006.
  • Extended lattice gas interactions of Cu on Cu (111) and Cu (001): Ab initio evaluation and implicationsTim John Stasevich, TL Einstein, Sergey Stolbov Physical Review B, 11, 2006.
  • Modeling the anisotropy of step fluctuations on surfaces: Theoretical step stiffness confronts experimentTim JohnStasevich University of Maryland Thesis, 2006.
  • Step fluctuations on Ag (111) surfaces with C 60Chenggang Tao, Tim John Stasevich, TL Einstein, ED Williams Physical Review B, 12, 2006.
  • Low-temperature orientation dependence of step stiffness on ${$111$}$ surfaces!!!Tim John Stasevich, Hailu Gebremariam, TL Einstein, M Giesen, C Steimer, H Ibach Physical Review B, 24, 2005.
  • Effects of next-nearest-neighbor interactions on the orientation dependence of step stiffness: Reconciling theory with experiment for Cu (001)Tim John Stasevich, TL Einstein, RKP Zia, M Giesen, H Ibach, F Szalma Physical Review B, 24, 2004.
  • Computation of charged-particle transfer maps for general fields and geometries using electromagnetic boundary-value dataParticle Accelerator Conference, 2001. PAC 2001. Proceedings of the 2001AJ Dragt, Tim John Stasevich, P Walstrom Proceedings of the 2001 Particle Accelerator Conference, 2001.