Tim Stasevich Assistant Professor

Office: Mrb 285

Phone: (000) 000-0000

Website: http://sites.bmb.colostate.edu/stasevichlab/

Google Scholar: https://scholar.google.com/citations?user=sGlZEDEAAAAJ&hl=en

Education

  • Ph.D., University of Maryland

About

Timothy J. Stasevich is an Assistant Professor in the Department of Biochemistry at Colorado State University (CSU). His lab uses a combination of advanced fluorescence microscopy, genetic engineering, and computational modeling to study the dynamics of gene regulation in living mammalian cells. Most recently, his lab has pioneered the imaging of real-time single mRNA translation dynamics in living cells [1]. Dr. Stasevich received his B.S. in Physics and Mathematics from the University of Michigan, Dearborn, and his Ph. D. in Physics from the University of Maryland, College Park. He transitioned into experimental biophysics as a post-doctoral research fellow in the laboratory of Dr. James G. McNally at the National Cancer Institute. During this time, he developed technology based on fluorescence microscopy to help establish gold-standard measurements of live-cell protein dynamics. Dr. Stasevich next moved to Osaka University, where he worked with Dr. Hiroshi Kimura as a Japan Society for the Promotion of Science Foreign Postdoctoral Research Fellow. While there, he helped create technology to image endogenous proteins and their post-translation modifications in vivo. This allowed him to image the live-cell dynamics of epigenetic histone modifications during gene activation for the first time [2]. Before joining the faculty at CSU, Dr. Stasevich took a one year hiatus at the HHMI Janelia Research Campus, where he further improved the spatio-temporal resolution of endogenous protein imaging in live-cells.

 

1.         Morisaki, T. et al. Real-time quantification of single RNA translation dynamics in living cells. Science 352, 1425–1429 (2016).

2.         Stasevich, T. J. et al. Regulation of RNA polymerase II activation by histone acetylation in single living cells. Nature 516, 272–275 (2014).

 

Publications

Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single geneLinda Stephanie Forero, Will Scott Raymond, Tetsuya Handa, Matthew Saxton, Tatsuya Morisaki, Hiroshi Kimura, Edouard Bertrand, Brian Munsky, Tim John Stasevich bioRxiv, 2020.
Author Correction: Quantifying the dynamics of IRES and cap translation with single-molecule resolution in live cells.Amanda Lynn Koch, L Aguilera, Tatsuya Morisaki, Brian Munsky, Tim John Stasevich Nature structural & molecular biology, 12, 2020.
Quantifying the dynamics of IRES and cap translation with single-molecule resolution in live cells.Amanda Lynn Koch, L Aguilera, Tatsuya Morisaki, Brian Munsky, Tim John Stasevich Nature structural & molecular biology, 12, 2020.
Lamin A/C Deficiency Enables Increased Myosin2 Bipolar Filament Ensembles Which Promote Divergent Actomyosin Network AnomaliesO'Neil P. Wiggan, Jennifer G. DeLuca, Tim John Stasevich, James R. Bamburg Molecular Biology of the Cell, 21, 2020.
Coupling of translation quality control and mRNA targeting to stress granules.Shaun Moon, Tatsuya Morisaki, Tim John Stasevich, R Parker The Journal of cell biology, 8, 2020.
Lighting up single-mRNA translation dynamics in living cells.Charlotte Ayn Cialek, Amanda Lynn Koch, Gabriel Galindo, Tim John Stasevich Current opinion in genetics & development, 2020.
A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivoNing Zhao, Kouta Kamijo, Philip Douglas Fox, Haruka Oda, Tatsuya Morisaki, Yuko Sato, Hiroshi Kimura, Tim John Stasevich NATURE COMMUNICATIONS, 2019.
Computational design and interpretation of single-RNA translation experimentsLuis U. Aguilera, William Raymond, Zachary R. Fox, Michael Patrick May, Elliot Djokic, Tatsuya Morisaki, Tim John Stasevich, Brian Munsky PLOS COMPUTATIONAL BIOLOGY, 10, 2019.
Live-Cell Single RNA Imaging Reveals Bursts of Translational FrameshiftingKenneth Ray Lyon, Luis U. Aguilera, Tatsuya Morisaki, Brian Munsky, Tim John Stasevich MOLECULAR CELL, 1, 2019.
Multicolour single-molecule tracking of mRNA interactions with RNP granulesStephanie L. Moon, Tatsuya Morisaki, Anthony Khong, Kenneth Ray Lyon, Roy Parker, Tim John Stasevich NATURE CELL BIOLOGY, 2, 2019.