Office: MRB 285
Phone: (970) 491-3250
Website: https://stasevichlab.colostate.edu
Google Scholar: https://scholar.google.com/citations?user=sGlZEDEAAAAJ&hl=en
Education
- Ph.D., University of Maryland
About
Timothy J. Stasevich is an Assistant Professor in the Department of Biochemistry at Colorado State University (CSU). His lab uses a combination of advanced fluorescence microscopy, genetic engineering, and computational modeling to study the dynamics of gene regulation in living mammalian cells. Most recently, his lab has pioneered the imaging of real-time single mRNA translation dynamics in living cells [1]. Dr. Stasevich received his B.S. in Physics and Mathematics from the University of Michigan, Dearborn, and his Ph. D. in Physics from the University of Maryland, College Park. He transitioned into experimental biophysics as a post-doctoral research fellow in the laboratory of Dr. James G. McNally at the National Cancer Institute. During this time, he developed technology based on fluorescence microscopy to help establish gold-standard measurements of live-cell protein dynamics. Dr. Stasevich next moved to Osaka University, where he worked with Dr. Hiroshi Kimura as a Japan Society for the Promotion of Science Foreign Postdoctoral Research Fellow. While there, he helped create technology to image endogenous proteins and their post-translation modifications in vivo. This allowed him to image the live-cell dynamics of epigenetic histone modifications during gene activation for the first time [2]. Before joining the faculty at CSU, Dr. Stasevich took a one year hiatus at the HHMI Janelia Research Campus, where he further improved the spatio-temporal resolution of endogenous protein imaging in live-cells.
1. Morisaki, T. et al. Real-time quantification of single RNA translation dynamics in living cells. Science 352, 1425–1429 (2016).
2. Stasevich, T. J. et al. Regulation of RNA polymerase II activation by histone acetylation in single living cells. Nature 516, 272–275 (2014).
Publications
- Using Mechanistic Models and Machine Learning to Design Single-Color Multiplexed Nascent Chain Tracking Experiments. bioRxiv : the preprint server for biology, 2023.
- Visualization, Quantification, and Modeling of Endogenous RNA Polymerase II Phosphorylation at a Single-copy Gene in Living Cells. Bio-protocol, 15, 2022.
- Imaging translational control by Argonaute with single-molecule resolution in live cells. Nature communications, 1, 2022.
- A Drosophila toolkit for HA-tagged proteins unveils a block in autophagy flux in the last instar larval fat body. Development (Cambridge, England), 6, 2022.
- Single-Molecule Imaging of mRNA Interactions with Stress Granules. Methods in molecular biology (Clifton, N.J.), 2022.
- Generation and diversification of recombinant monoclonal antibodies Elife, 2021.
- Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells. Genes to cells : devoted to molecular & cellular mechanisms, 11, 2021.
- A Multi-color Bicistronic Biosensor to Compare the Translation Dynamics of Different Open Reading Frames at Single-molecule Resolution in Live Cells. Bio-protocol, 14, 2021.
- Bead Loading Proteins and Nucleic Acids into Adherent Human Cells. Journal of visualized experiments : JoVE, 172, 2021.
- Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene. Nature communications, 1, 2021.
- Protein manipulation using single copies of short peptide tags in cultured cells and in Drosophila melanogaster. Development (Cambridge, England), 6, 2021.
- Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene bioRxiv, 2020.
- Author Correction: Quantifying the dynamics of IRES and cap translation with single-molecule resolution in live cells. Nature structural & molecular biology, 12, 2020.
- Quantifying the dynamics of IRES and cap translation with single-molecule resolution in live cells. Nature structural & molecular biology, 12, 2020.
- Lamin A/C Deficiency Enables Increased Myosin2 Bipolar Filament Ensembles Which Promote Divergent Actomyosin Network Anomalies Molecular Biology of the Cell, 21, 2020.
- Coupling of translation quality control and mRNA targeting to stress granules. The Journal of cell biology, 8, 2020.
- Lighting up single-mRNA translation dynamics in living cells. Current opinion in genetics & development, 2020.
- A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo NATURE COMMUNICATIONS, 2019.
- Computational design and interpretation of single-RNA translation experiments PLOS COMPUTATIONAL BIOLOGY, 10, 2019.
- Live-Cell Single RNA Imaging Reveals Bursts of Translational Frameshifting MOLECULAR CELL, 1, 2019.
- Multicolour single-molecule tracking of mRNA interactions with RNP granules NATURE CELL BIOLOGY, 2, 2019.
- Satb1 integrates DNA binding site geometry and torsional stress to differentially target nucleosome-dense regions NATURE COMMUNICATIONS, 2019.
- Probing Cytoskeletal mechanics and heterogeneities using glass-bead-loading to deliver 100 nm fluorescent particles into mammalian cells Soft Matter.
- Quantifying single-mRNA translation kinetics in living cells Cold Springs Harbor Perspectives in Biology, 11, 2018.
- Visualizing the Dynamics of Inactive X Chromosomes in Living Cells Using Antibody-Based Fluorescent Probes X-Chromosome Inactivation: Methods in Molecular Biology, 2018.
- Imaging Translational and Post-Translational Gene Regulatory Dynamics in Living Cells with Antibody-Based Probes TRENDS IN GENETICS, 5, 2017.
- She1 affects dynein through direct interactions with the microtubule and the dynein microtubule-binding domain Nature Communications, 1, 2017.
- Real-time quantification of single RNA translation dynamics in living cells Science, 6292, 2016.
- Estimating cellular parameters through optimization procedures: elementary principles and applications Frontiers in physiology, 2015.
- High-performance probes for light and electron microscopy Nature methods, 6, 2015.
- Visualizing posttranslational and epigenetic modifications of endogenous proteins in vivo Histochemistry and cell biology, 2, 2015.
- Evaluation of chemical fluorescent dyes as a protein conjugation partner for live cell imaging PloS one, 9, 2014.
- Quantifying histone and RNA polymerase II post-translational modification dynamics in mother and daughter cells Methods, 2, 2014.
- Regulation of RNA polymerase II activation by histone acetylation in single living cells Nature, 7530, 2014.
- Convergence of chromatin binding estimates in live cells Nature methods, 8, 2013.
- Genetically encoded system to track histone modification in vivo Scientific reports, 2013.
- Quantifying transcription factor kinetics: at work or at play? Critical reviews in biochemistry and molecular biology, 5, 2013.
- Single-cell in vivo analysis reveals that relocation of the yeast CUP1 locus to the nuclear pore is associated with its mRNA releaseYEAST Yeast, 2013.
- Monitoring dynamic binding of chromatin proteins in vivo by fluorescence correlation spectroscopy and temporal image correlation spectroscopy Chromatin Remodeling: Methods and Protocols, 2012.
- A selection and analysis tool for fluorescence recovery after photobleaching (FRAP) region of interest (ROI) Wolfram Demonstration Project, 2011.
- Assembly of the transcription machinery: ordered and stable, random and dynamic, or both? Chromosoma, 6, 2011.
- Highly stable loading of Mcm proteins onto chromatin in living cells requires replication to unload The Journal of cell biology, 1, 2011.
- Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labeling Nucleic acids research, 2011.
- Core histone hyperacetylation impacts cooperative behavior and high-affinity binding of histone H1 to chromatin Biochemistry, 21, 2010.
- Cross-validating FRAP and FCS to quantify the impact of photobleaching on in vivo binding estimates Biophysical journal, 9, 2010.
- Dissecting the binding mechanism of the linker histone in live cells: an integrated FRAP analysis The EMBO journal, 7, 2010.
- Epitaxial growth writ large Science, 5964, 2010.
- FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know? Current opinion in cell biology, 3, 2010.
- Direct measurement of association and dissociation rates of DNA binding in live cells by fluorescence correlation spectroscopy Biophysical journal, 1, 2009.
- Effects of impurities on surface morphology: some examples Journal of Physics: Condensed Matter, 8, 2009.
- Effects of impurities on surface morphology: some examples. Journal of physics. Condensed matter: an Institute of Physics journal, 8, 2009.
- Impurity decoration for crystal shape control: C 60 on Ag (111) Physical review letters, 8, 2009.
- Concurrent fast and slow cycling of a transcriptional activator at an endogenous promoter Science, 5862, 2008.
- Sensitivity of short-range trio interactions to lateral relaxation of adatoms: Challenges for detailed lattice-gas modeling Surface Science, 6, 2008.
- Step line tension and step morphological evolution on the Si (111)(1$$ 1) surface!!! Physical Review B, 11, 2008.
- Analytic formulas for the orientation dependence of step stiffness and line tension: Key ingredients for numerical modeling Multiscale Modeling & Simulation, 1, 2007.
- Facet-edge fluctuations with periphery diffusion kinetics Surface Science, 18, 2007.
- Metal-molecule interface fluctuations Nano letters, 6, 2007.
- Distinctive fluctuations in a confined geometry Physical review letters, 8, 2006.
- Extended lattice gas interactions of Cu on Cu (111) and Cu (001): Ab initio evaluation and implications Physical Review B, 11, 2006.
- Modeling the anisotropy of step fluctuations on surfaces: Theoretical step stiffness confronts experiment University of Maryland Thesis, 2006.
- Step fluctuations on Ag (111) surfaces with C 60 Physical Review B, 12, 2006.
- Low-temperature orientation dependence of step stiffness on ${$111$}$ surfaces!!! Physical Review B, 24, 2005.
- Effects of next-nearest-neighbor interactions on the orientation dependence of step stiffness: Reconciling theory with experiment for Cu (001) Physical Review B, 24, 2004.
- Computation of charged-particle transfer maps for general fields and geometries using electromagnetic boundary-value dataParticle Accelerator Conference, 2001. PAC 2001. Proceedings of the 2001 Proceedings of the 2001 Particle Accelerator Conference, 2001.