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Tingting Yao
Tingting Yao Professor

Office: MRB 283

Phone: (970) 492-4116

Google Scholar: https://scholar.google.com/citations?user=y4x3LGIAAAAJ&hl=en

Education

  • B.S. Wuhan University, China
  • Ph.D. University of Iowa, Iowa City
  • Postdoc. Stowers Institute for Medical Research

About

Our lab studies the interface between the ubiquitin-proteasome pathway and chromatin regulation. Modification of components of chromatin and its associated machinery by ubiquitin can serve as a regulatory switch that have diverse functions in transcription and DNA repair. We are using a variety of biochemical and cell biology approaches to define the molecular mechanisms that regulate the dynamics of ubiquitin conjugation and deconjugation in these processes.

Ubiquitin (Ub) is a small protein of 76 amino acids. It is ubiquitous among all eukaryotes and it is highly conserved from yeast to man. The covalent attachment of Ub to other proteins, i.e., ubiquitination, is used as a regulatory signal in a broad spectrum of biological processes. These include targeting proteins for proteasomal degradation, intracellular trafficking, transcription activation, DNA damage repair, and chromatin remodeling. These different outcomes are due in part to the versatility of the Ub modification, which can vary in the number of Ub molecules attached and, if polyubiquitin chains are formed, the linkage between ubiquitins in the chain. Moreover, like phosphorylation, ubiquitination is reversible. Proteins are subject to ubiquitination and deubiquitination, and the balance between these competing processes determines the fate of the substrate. Although histones were among the first proteins found to be ubiquitinated, it was demonstrated only recently that ubiquitinated histone H2A is a hallmark of repressed chromatin, and that dynamic ubiquitination and deubiquitination of histone H2B is important for gene activation. We are interested in how these diverse ubiquitin signals are written, erased, and read by the cellular machinery.

Publications