Office: Az E206F
Phone: (000) 000-0000
Google Scholar: https://scholar.google.com/citations?user=xxf7aHAAAAAJ&hl=en
I share my passion for science and strive to instill principles of scientific integrity, collegiality, professionalism and the drive to succeed. In LIFE 212, students learn the basic scientific skills of data collection, interpretation, critical thinking, and writing, all while learning the experimental methods and technology that are commonly used in cell and molecular biology research labs.
Research- Mechanisms of mosquito resistance to insecticides/ RVFV vaccine safety studies
Association mapping of factors that condition pyrethroid resistance in Aedes aegypti has consistently identified genes in multiple functional groups. Toward better understanding of the mechanisms involved in resistance, we mined association mapping data to identify additional genes to add the voltage-gated sodium channel (VGSC) interactome, which is the target of pyrethroids. The RNA interference (RNAi) pathway uses miRNAs to regulate gene expression critical to development and cellular homeostasis. RNA-induced silencing protein complexes (miRISCs) bind to miRNAs and use them to identify and inhibit target protein translation via miRNAi. In addition, miRNAi can also act through the RNA decay pathway to target mRNA silencing. We found that, in Aedes aegypti, miR-33 modulates the insecticide permethrin target (VGSC) and also that strain-specific differences in Vgsc levels are associated with permethrin susceptibility.
In another project, I am also working to validate a new vaccine for Rift Valley Fever virus (RVFV). RVFV is a dangerous zoonotic mosquito-borne pathogen that periodically emerges in epizootics as shown above. It causes birth defects and abortion in sheep and cattle. When spread to humans, it causes fever in most, but can lead to encephalitis, kidney damage, eye damage, severe anemia and hemorrhagic fever. CDC scientists deleted two genome segments (NSs and NSm) to generate a recombinant vaccine strain. This strain is safe, immunogenic and confers protection against teratogenesis and abortion in pregnant sheep. Further testing in mosquitoes showed that deletion of NSm severely restricted mosquito midgut infection and escape and abrogated virus transmission. This vaccine strain is being prepared by BioMarc at CSU in collaboration with the Coalition for Epidemic Preparedness Innovations (CEPI). We will determine if mosquitoes are capable of replicating and transmitting the live vaccine strain (DDVax) after biting vaccinated goats.
I use my maiden name, CL Campbell, in publications.