Six research groups in the department are working to unravel the biophysical basis of complex biological events spanning from the cell surface to the nucleus. The research includes detailed structural studies on chromatin and transcription complexes and the protein-protein interactions involved in the cell cycle. Other research projects delve into the biophysical mechanism of protein folding, stability and design, and the basic methodologies that are used in the determination of biomolecular structure and dynamics. X-ray diffraction, analytical ultracentrifugation, and optical spectroscopic methods are being used to evaluate the structure and dynamics of the multi-component molecular machines that are central to the processes of gene-regulation, energy interconversion and signalling, and cell-cycle regulation.
Effect of Acidosis on Renal Gene Expression: Dr. Curthoys
Reconstitution of Chromatin; Chromatin Structure and the Regulation of Gene Expression: Dr. Hansen, Dr. Stargell
Transcriptional Activation by the Human T-Cell Leukemia Virus Tax Protein; Involvement of Coactivators and Tumor Suppressors: Dr. Nyborg
Mechanisms of Transcriptional Initiation by RNA Polymerase II: Dr. Stargell
Regulation of chromatin structure and dynamics by histone ubiquitination: Dr. Tingting Yao
Cellular biochemistry focuses on the signaling processes that permit the elaborate cell-cell communication required in a multicellular organism. Five research groups are currently studying cellular biochemistry, focusing their research on the rapid changes in cytoskeletal proteins, protein kinases, membrane lipids, and small ions that accompany extracellular signals. These groups utilize a broad array of biochemical techniques including fluorescence and electron microscopy, gene expression, and electrophysiology to characterize these fundamental biological processes.
The Role of ADF in Neural Degeneration: Dr. Bamburg
Mechanisms of Mitotic Chromosome Segregation Dr. DeLuca
Regulation of Neuronal Excitability: Dr. Chanda
Retrovirus Assembly and Budding
Regulation of ADF/Cofilin Proteins: Dr. Bamburg
Ubiquitin metabolism and ubiquitin-mediated signaling