Grant Schauer Assistant Professor

Office: Mrb 231

Phone: (970) 491-2695

Education

  • Ph.D. Molecular Biophysics and Structural Biology, University of Pittsburgh and Carnegie Mellon University
  • M.S. Biomedical Sciences, University of New Mexico
  • B.A. Biochemistry, University of New Mexico

About

The replisome is a multiprotein machine that orchestrates duplication of the genome—including the epigenome—with strikingly high accuracy despite a constant barrage of obstacles. These challenges to replication pose risks for mutagenesis and replication fork collapse. My lab is interested in auxiliary machinery that directly couples to the core replisome, handling these obstacles in coordination with the DNA replication apparatus to achieve high fidelity chromosome duplication. Using replisomes and chromatin reconstituted from protein complexes purified in yeast, we study these molecular mechanisms in detail with single-molecule fluorescence techniques in addition to traditional biochemistry.

Publications

Tunability of DNA Polymerase Stability During Eukaryotic ReplicationLewis JS†, Spenkelink LM†, Schauer GD, Yurieva O, Natarajan V, Kaur G, Maher C, Kay C, O’Donnell ME, van Oijen AM.Molecular Cell, 2020 Jan 2; 77(1): 17-25.e5. †co-first author,
Replisome preservation by a single-stranded DNA gate in the CMG helicaseWasserman MR†, Schauer GD†, O’Donnell ME*, Liu S*Cell, 2019 Jul 25;178(3):600-611.e16. †co-first author; *co-corresponding author,
Nuclease dead Cas9 is a programmable roadblock for DNA replicationWhinn K, Kaur G, Lewis, JS, Schauer GD, Müller S, Jergic S, Naganbabu M, Bruchez M, O’Donnell ME, Dixon N, van Oijen AM, Ghodke H.Sci Rep. 2019 Sep 16;9(1):13292, 2019
Single-molecule visualization of Saccharomyces cerevisiae leading-strand synthesis reveals dynamic interaction between MTC and the replisomeLewis JS†, Spenkelink LM†, Schauer GD, Hill FR, Georgescu RE, O’Donnell ME, van Oijen AMProc Natl Acad Sci USA. 2017 Oct 3;114(40):10630–10635. PMCID: PMC5635917; †co-first author,
Mcm10 promotes rapid isomerization of CMG-DNA for replisome bypass of lagging strand DNA blocksLangston LD, Mayle R, Schauer GD, Yurieva O, Zhang D, Yao NY, Georgescu RE, O’Donnell ME.eLife. 2017 Sep 4;6. PMCID: PMC5599239,
Reconstitution of a eukaryotic replisome reveals suppression mechanisms that define leading/lagging strand operationGeorgescu RE, Schauer GD, Yao NY, Langston LD, Yurieva O, Zhang D, Finkelstein J, O’Donnell ME. eLife. 2015 Apr 30. PMCID: PMC4413876,
Mechanism of allosteric inhibition of HIV-1 reverse transcriptase revealed by single-molecule and ensemble fluorescenceSchauer GD, Huber KD, Leuba SH, Sluis-Cremer N.Nucleic Acids Research. 2014 Oct;42(18):11687–11696. PMCID: PMC4191400,
Early integration of the individual student in academic activities: a novel classroom concept for graduate education in molecular biophysics and structural biology. Leuba SH, Carney SM, Dahlburg EM, Eells RJ, Ghodke H, Yanamala N, Schauer GD, Klein-Seetharaman J. BMC Biophysics. 2014;7:6. PMCID: PMC4134111,
PcrA-mediated disruption of RecA nucleoprotein filaments--essential role of the ATPase activity of RecAFagerburg MV, Schauer GD, Thickman KR, Bianco PR, Khan SA, Leuba SH, Anand SP.Nucleic Acids Research. 2012;40(17):8416-24. PMCID: PMC3458574,
Steric exclusion and wrapping of the excluded DNA strand occurs along discrete external binding paths during MCM helicase unwindingGraham BW, Schauer GD, Leuba SH, Trakselis MA.Nucleic Acids Research. 2011;39(15):6585-95. PMCID: PMC3159478,