Person – Department of Biochemistry and Molecular Biology

Office:

Phone: (970) 491-0420

Website: https://swygertlab.colostate.edu/

Curriculum Vitae: https://swygertlab.colostate.edu/wp-content/uploads/2022/07/20220722_Swygert_CV.pdf

Google Scholar: https://scholar.google.com/citations?user=wbTK_a8AAAAJ&hl=en

Education

Postdoctoral training, Fred Hutchinson Cancer Research Center
Ph.D., University of Massachusetts Medical School
B.A., Agnes Scott College

About

The organization of genomes into chromatin controls all DNA-dependent processes. Recently, tremendous effort has been put into understanding how chromatin folds into increasingly large and complex three-dimensional structures. However, attributing biological functions to chromatin structures has been challenging. In my lab, we use a combination of the highest-possible resolution genomics methods and biochemical and biophysical assays to uncover the roles of 3D chromatin structures in cells. As a model, we use budding yeast in a mysterious cell state called quiescence, in which chromatin undergoes dramatic rearrangements and the majority of transcription is silenced. Our results have broad implications for basic regulatory mechanisms as well as specific applications to processes governed by quiescence.

Publications

Local chromatin fiber folding represses transcription and loop extrusion in quiescent cellsSwygert, S.G.,* Lin, D., Portillo-Ledesma, S., Lin, P.Y., Hunt, D.R., Kao, C.F., Schlick, T., Noble, W.S., Tsukiyama, T.* *Corresponding authorseLife 10:e72062, 2021
Unraveling quiescence-specific repressive chromatin domainsSwygert, S.G., Tsukiyama, T. Current Genetics 65, 1145-1151, 2019
Condensin-dependent chromatin compaction represses transcription globally during quiescenceSwygert, S.G., Kim, S., Wu, X., Fu, T., Hsieh, T.H., Rando, O.J., Eisenman, R.N., Shendure, J., McKnight, J.N., Tsukiyama, T.Molecular Cell 73, 533-546, 2019
SIR proteins create compact heterochromatin fibersSwygert, S.G., Senapati, S., Bolukbasi, M.F., Wolfe, S.A., Lindsay, S., Peterson, C.LProceedings of the National Academy of Sciences 115, 12447-12452, 2018
Preparation and analysis of Saccharomyces cerevisiae quiescent cellsSpain M.M., Swygert S.G., Tsukiyama T. Lacorazza H. (eds) Cellular Quiescence. Methods in Molecular Biology, vol 1686. Humana Press, New York, NY , 2018
Nucleosome-like, single-stranded DNA (ssDNA)-histone octamer complexes and the implication for DNA double strand break repairAdkins, N.L., Swygert, S.G., Kaur, P., Niu, H., Grigoryev, S.A., Sung, P., Wang, H., Peterson, C.L.Journal of Biological Chemistry 292, 5271-5281, 2017
Chromatin dynamics: interplay between remodeling enzymes and histone modificationsSwygert, S.G. & Peterson, C.LBiochimica et Biophysica Acta - Gene Regulatory Mechanisms 1839, 728-736, 2014
Solution-state conformation and stoichiometry of yeast Sir3 heterochromatin fibresSwygert, S.G., Manning, B.J., Senapati, S., Kaur, P., Lindsay, S., Demeler, B., Peterson, C.L. Nature Communications 5, 4751, 2014

Colorado State University

College of Natural Sciences

Publications