Office: MRB 233
Phone: (970) 491-0726
- PhD, Purdue University
- MS, Chinease Academy of Science, Institute of Microbiology
Retroviruses are enveloped RNA viruses, which comprise a large and diverse family including human immunodeficiency virus 1 (HIV-1), the causitive pathogen of AIDS. During the late stage of virus replication, specific and highly concerted interactions among viral components and host cofactors are required for progeny virion assembly and release. An exciting possibility is that these specific virus-host interfaces might represent novel targets for the development of preventative or therapeutic strategies against HIV-1 infection.
We are interested in characterization of the molecular and cellular mechanisms underlying this late stage of HIV-1 replication using a combination of biological imaging, cellular and molecular virology approaches. The ongoing projects include: 1) Understanding the mechanism of HIV-1 protease autoprocessing, a viral specific reaction responsible for production of the mature protease; 2) Identification and characterization of molecules selectively interfering with protease autoprocessing. We have established a functional assay that allows direct quantification of protease autoprocessing in cells. This assay will enable an unprecedented screen for small molecule inhibitors that suppress autoprocessing via a novel interference mechanism.
- Targeting HIV-1 Protease Autoprocessing for High-throughput Drug Discovery and Drug Resistance AssessmentSci Rep, 9:301, 2019
- Context-dependent Autoprocessing of Human Immunodeficiency Virus Type 1 Protease PrecursorsPLoS One, 13:e0191372, 2018
- The HIV-1 Late Domain-2 S40A Polymorphism in Antiretroviral (or ART)-exposed Individuals Influences Protease Inhibitor SusceptibilityRetrovirology, 13:64, 2016
- A Functional Interplay between Human Immunodeficiency Virus Type 1 Protease Residues 77 and 93 Involved in Differential Regulation of Precursor Autoprocessing and Mature Protease ActivityPLoS One, 10:e0123561, 2015
- Understanding HIV-1 Protease Autoprocessing for Novel Therapeutic DevelopmentFuture Med Chem, 5:1215, 2013
- Flexible Catalytic Site Conformations Implicated in Modulation of HIV-1 Protease Autoprocessing ReactionsRetrovirology 8:79, 2011
- Modulation of Human Immunodeficiency Virus Type 1 Protease Autoprocessing by Charge Properties of Surface Residue 69J Virol 83:7789, 2009
- Functions of Early (AP-2) and Late (AIP1/ALIX) Endocytic Proteins in Equine Infectious Anemia Virus BuddingJ Biol Chem 280:40474, 2005
- Differential Effects of Actin Cytoskeleton Dynamics on Equine Infectious Anemia Virus Particle ProductionJ Virol 78:882, 2004
- Characterization of RNA Elements That Regulate Gag-Pol Ribosomal Frameshifting in Equine Infectious Anemia VirusJ Virol 77:10280, 2003